Biological Control of Dengue mosquito (Aedes aegypti)Use Entomopathogenic Nematodes (Romanomermis culicivorax)

Biological Control of Dengue mosquito (Aedes aegypti) in the Capital Region Use Entomopathogenic Nematodes (Romanomermis culicivorax) Safe Use of the Community and Environment


Name: Lutfi Afifah (Nickname: Lutfi)
Addres: Wisma Taruna Jl. Babakan Raya No. 20 Rw 07, Rt 04

Darmaga Bogor 16680 Jawa Barat

Telp: 085235770050 Email: lutfiafifah@ymail.com
University: Bogor Agricultur University
Faculty: Agriculture Departement: Plant Protection

I.TITLE

Biological Control of Dengue mosquito (Aedes aegypti) in the Capital Region Use Entomopathogenic Nematodes (Romanomermis culicivorax) Safe Use of the Community and Environment.

II.SUMMARY
(Summary from the idea that events are contested)

Activities that will be done of controlling dengue fever mosquito Aedes aegypti using a biological agent that will be done together communities in the region capital of Jakarta. Initially activities will begin with rearing R. culicivorax in Nematology laboratory, Bogor Agricultural University. Next is a correctional consisting of (socialization, campaigns, training, and supervision) to the public on the importance of dengue mosquito control insecticides naturally without chemicals so the environment and public health can be maintained.

III.INTRODUCTION
(Containing the framework and process of the ideas that will be done)

Starting in January up to March 5th 2004, total cases of dengue fever (DHF) in all provinces in Indonesia have reached 26 015, with as many as 389 people the number of deaths (CFR = 1.53%). Have the highest cases in DKI Jakarta (11 534 persons) while the highest CFR contained in NTT Province (3.96%).

Dengue Fever or Dengue hemorrhagic fever (DHF) is a disease caused by dengue virus is transmitted through mosquito bites A. aegypti and A. albopictus. Both mosquito species have almost all corners of Indonesia, except in places a height of more than 1000 meters above sea level. Increased number of cases and the increase in the affected areas, due to the better transport of the population, the existence of new settlements, lack of community behavior toward cleaning mosquito breeding, and the presence of mosquito vectors in almost all corners of the country. During the time for mosquito control A. aegypti and Anopheles, there are still many people who use chemicals (insecticide). In fact, it’s not environmentally friendly and already there are indications of resistance of mosquitoes A. aegypti and Anopheles at various places on certain types of insecticides. Once insekticide materials that go into the ground, it will be carried by rain water and will be collected in one place. Mosquitoes will breed in there and will adapt to the environment so that resistance will emerge.

Society seems less concerned with the environment, so that the environment alone would be bad for society, if not guarded existence. Communities around the slums, especially in Jakarta, the outskirts of the river is generally never be devoid of human activity that comes and goes out the trash, bathing, washing, or even take water from these rivers for consumption. Though the water is not necessarily feasible for use. This is certainly going to aggravate the condition of public health. Thus the need for public knowledge about environmental health needs to be encouraged again.
Proposed program is to control the dengue mosquitoes biologically so that there is dengue, especially in the capital could be reduced. In addition to further promote environmentally friendly control concept to the public. To further hoped that the existence of a sustainable control environment and public health can be maintained.

At present, essentially all of the available information relating to use of mermithids for the control of insects has been generated from studies with R. culicivorax. Thus, R. culicivorax serves as a model system for other aquatic mermithid species as their mass production systems are developed. Studies with this species have shown the potential for either inundative control systems, that is, the release of infective stages in sufficient numbers to give immediate control of mosquito populations, or inoculative control, that is, the introduction of the postparasitic stage or reduced numbers in the preparasitic stage to establish the nematode population in the environment to give partial control for an indefinite period. Studies with R. culicivorax have also demonstrated the limitations associated with the use of agents of this type. As living organisms, a knowledge of limiting physical and chemical factors is essential if the parasite is to be effectively used. Without this knowledge potentially effective agents may be discarded because they were used in environments outside their limits of tolerance.

Live cycle of R. culicivorax

Mermithids (especially R. culicivorax) have drawn attention because they possess the following characteristics of an ideal biological control agent: (1) they are host specific to one or a few species of hosts; (2) parasitism is usually lethal to host; (3) they are generally easy to manipulate in the laboratory; (4) some can be mass produced; (5) they are easily disseminated in the environment with standard pesticide application techniques; (6) they have the potential for establishment and recycling and give control for extended periods; and (7) they present no environmental threat. Conversely, many of the desired characteristics of these agents make them unattractive for commercial development. For example, host specificity, a characteristic that gives mermithids a unique advantage over chemical pesticides, also limts their usefulness, to at most, only a few target insects. This means that the total product volume sold per year will be considerably less than would be sold if the product were applicable to a range of pests. Also, other characteristics such as the potential for establishment and recycling further threaten to reduce the potential market for a mermithid product in the eyes of prospective producers. In addition, the problems of environmental limitations on the mermithid further reduce the potential effective use of a given mermithid species.

IV. PURPOSE

(The purpose of the program to be conducted)

Dengue mosquito control program aims to be able to suppress the development of mosquito A. aegypti biologically. So hopefully it can reduce dependence on chemical control by using insecticides. Moreover, it can reduce mortality due to dengue fever, particularly in the capital Jakarta, and made a model village free of litter and free of dengue mosquitoes.

V. ACTIVITY PROGRAM

(Described in detail the activities to be undertaken in connection with the objectives to be achieved [location, perpetrators executors, beneficiaries, etc.])

In a biological mosquito control activities for dengue fever, the previous test the effectiveness of R. culicivorax will held in Nematology laboratory, Department of Plant Protection, Faculty of Agriculture, Bogor Agricultural University. In this test will see the effectiveness of R. culicivorax in suppressing the growth and development of A. aegypti. Another thing that will be observed is the calculation of the number of entomopathogenic nematodes R. culicivorax will infestation in the field as biological control. In this activity, I will also do a campaign of socialization and R. culicivorax as mosquito control agents of dengue fever to the population residing in slum areas in Jakarta in cooperation with a local villager and some primary schools to gain knowledge about the utilization of this biological agent. Implementing these activities I will submit proposal to several NGOs working in the field of environment and in cooperation with the Jakarta city government. Next look for agencies engaged in Nematology who are willing to hold a mass breeding R. culicivorax for dengue mosquito control safe for the environment. Then make an application with menginfestasikan R. culicivorax in places which are indicated as mosquito breeding places of dengue fever. This application activities conducted jointly mass society and re-done in a sustainable breeding. In this application activities will give reward for the people who managed to do a mass breeding and the application properly and correctly.

Benefits from this event given the community will be able to enjoy the area will be protected from the dangers of dengue fever. Moreover, it can reduce the pollution caused by waste and can reduce the environmental damage caused by the use of chemical insecticides are very dangerous for public safety and the environment.

VI. ACHIEVE PRODUCT

(A result that can be measured by specific, measurable, achievable, can be accounted for within a certain time)

Specifically mosquito control dengue fever in Jakarta, especially in slum areas will be able to provide an alternative environment-friendly controls that will suppress the growth of mosquito A. aegypti. The results showed the success of many mosquito A. aegypti in the field by R culicivorax that parsitism. So the program will run well.

VII. BENEFIT ACTIVITY
(Benefit activities for the environment, economic and social)

Environment

Biological control using entomopathogenic nematodes must be able to reduce and suppress the dengue fever mosquito, especially in the capital Jakarta, which in turn helps the environment to be able to return to the ecosystem balance, reduce reliance on insecticides chemical environments, as well as encourage the formation of a sustainable environment and sustainable development.

Economy

Communities can increase their earnings is by making the formulation of entomopathogenic nematodes R. culicivorax which can be used as biological control without the high cost. In addition, if control of dengue mosquitoes in an area worked well and added a nice arrangement of the village hall will be able to make an area / village to “Model Village”. Therefore, expected to become a pilot village area that will become one of tourist destinations. Thereby increasing incomes.

Social

Communities can cooperate in biological control dengue fever mosquito, except it will educate children to love the environment and protecting the environment to remain sustainable.

VIII. TIME

(Target time execution)

This control program will be done gradually starting from a mass breeding and testing the effectiveness of R. culicivorax on A. aegypti to be carried out in laboratories Nematology, IPB. Furthermore, socialization, and a campaign against the community and elementary school students in a slum in the capital city of Jakarta. Courses will be conducted within approximately three months.

IX. COST
(Approximate dibutukan funds in conducting these activities)

No. Description Number of units Units Per unit (Rp) Total
Mass breeding R. culicivorax
1 Bucket / fruit tray 15 pack 3000 60000
2 Cotton 2 pack 10000 20000
3 Lease of one semester of laboratory * 1 semester 70000 70000
4 The filter mesh * 1 pack 20000 20000
5 Kassa 10 meter 3000 30000
6 Sand / soil bags 1 bag 50000 50000
Campaign activities and socialization
7 making leaflet 1 copies 10000 10000
8 making poster 1 copies 35000 35000
9 multiplication leaflet 50 copies 1500 75000
10 multiplication posters 10 copies 5000 50000
11 Transportation 50000
Total 470000

* Possible it will be facilitated by the Nematology laboratory, Bogor Agricultural University, so it does not require a fee.

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